Metagenomics workshop - wetlab agenda

Home / Wetlab Agenda

Metagenomic Wetlab Session Agenda



Day 1 (Monday, August 19, 2019):

Metagenome sequencing: workflow and library preparation
Time Title Session Lead
9:30 – 10:00
Registration

  • Get name tags
  • Course material

Janine McCalder
10:00 – 10:15 Welcoming Remarks

Dr. Marc Strous
10:15 – 10:45
Workflow of metagenome library prep and sequencing on the MiSeq

  • Overview of the metagenome kit
  • Sequencing run output

Carmen Li

Dr. Christine Sharp
10:45 – 11:00 Lab orientation and safety

Carmen Li

Dr. Christine Sharp
11:00 – 12:00 Lunch
12:00 - 16:00
Metagenome library preparation

  • Workstation setup and introduction
  • Distribution of reagent aliquots
  • Tagmentation
  • Cleanup
  • Amplification

Carmen Li

Dr. Christine Sharp


Day 2 (Tuesday, August 20, 2019):

Metagenome library selection, loading Miseq, and trouble shooting
Time Title Sesson Lead
9:30 – 9:45
Quick overview of the program for the day

 

Carmen Li

Dr. Christine Sharp
9:45-11:00
Metagenome library preparation (continued)

  • Library fragment size check by bioanalyzer
  • Quantification of DNA using Qubit
  • Discussion of bioanalyzer data

Carmen Li

Dr. Christine Sharp
11:00 – 12:00 Lunch
12:00 - 13:30
Metagenome library preparation (continued)

  • Selection of three metagenome libraries for sequencing
  • Normalizing and pooling libraries
  • Demo of prepping, loading, and running the Illumina MiSeq

Carmen Li

Dr. Christine Sharp
13:30 - 15:00
Troubleshooting metagenome library prep.

  • Presentation on troubleshooting metagenome library prep and sequencing
  • Case studies
  • Q&A session

Carmen Li

Dr. Christine Sharp
15:00 – 15:30 Closing Remarks

Dr. Marc Strous