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Metagenomic Wetlab Session Agenda

Day 1 (Monday, August 19, 2019):

Metagenome sequencing: workflow and library preparation
Time	Title	Session Lead
9:30 – 10:00	Registration Get name tags Course material	Janine McCalder
10:00 – 10:15	Welcoming Remarks	Dr. Marc Strous
10:15 – 10:45	Workflow of metagenome library prep and sequencing on the MiSeq Overview of the metagenome kit Sequencing run output	Carmen Li Dr. Christine Sharp
10:45 – 11:00	Lab orientation and safety	Carmen Li Dr. Christine Sharp
11:00 – 12:00	Lunch
12:00 - 16:00	Metagenome library preparation Workstation setup and introduction Distribution of reagent aliquots Tagmentation Cleanup Amplification	Carmen Li Dr. Christine Sharp

Day 2 (Tuesday, August 20, 2019):

Metagenome library selection, loading Miseq, and trouble shooting
Time	Title	Sesson Lead
9:30 – 9:45	Quick overview of the program for the day	Carmen Li Dr. Christine Sharp
9:45-11:00	Metagenome library preparation (continued) Library fragment size check by bioanalyzer Quantification of DNA using Qubit Discussion of bioanalyzer data	Carmen Li Dr. Christine Sharp
11:00 – 12:00	Lunch
12:00 - 13:30	Metagenome library preparation (continued) Selection of three metagenome libraries for sequencing Normalizing and pooling libraries Demo of prepping, loading, and running the Illumina MiSeq	Carmen Li Dr. Christine Sharp
13:30 - 15:00	Troubleshooting metagenome library prep. Presentation on troubleshooting metagenome library prep and sequencing Case studies Q&A session	Carmen Li Dr. Christine Sharp
15:00 – 15:30	Closing Remarks	Dr. Marc Strous