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Metagenomic Wetlab Session Agenda

Day 1 (Wednesday, June 20, 2018):

Metagenome sequencing: workflow and library preparation
Time	Title	Session Lead
8:30 – 9:00	Registration Get name tags Course material
9:00 – 9:15	Welcoming Remarks	Dr. Marc Strous
9:15 – 10:45	Workflow of metagenome library prep and sequencing on the MiSeq Overview of the KAPA metagenome kit Workings of indexing libraries Sequencing run output	Carmen Li Dr. Christine Sharp
10:45 – 11:00	Lab orientation and safety	Carmen Li Dr. Christine Sharp
11:00 – 12:00	Lunch
12:00 - 17:00	Metagenome library preparation Workstation setup and introduction Distribution of reagent aliquots Enzymatic fragmentation End repair and A-tailing Adapter ligation Post-ligation cleanup	Carmen Li Dr. Christine Sharp

Day 2 (Thursday, June 21, 2018):

Metagenome library preparation
Time	Title	Sesson Lead
8:30 – 8:45	Quick overview of the program for the day	Carmen Li Dr. Christine Sharp
8:45-12:30	Metagenome library preparation (continued) Library amplification Size selection of DNA fragments using magnetic beads Library fragment size check by bioanalyzer	Carmen Li Dr. Christine Sharp
12:30 – 13:30	Lunch
13:30 - 16:30	Metagenome library preparation (continued) Quantification of DNA using Qubit Selection of three metagenome libraries for sequencing Normalizing and pooling libraries	Carmen Li Dr. Christine Sharp

Day 3 (Friday, June 22, 2018):

Metagenome library selection, loading Miseq, and trouble shooting
Time	Title	Session Lead
8:45 – 9:00	Quick overview of the program for the day	Carmen Li Dr. Christine Sharp
9:00 – 10:00	Illumina presentation: Illumina sequencing technology overview	Dr. Shruti Parikh
10:00 - 11:00	Metagenome library preparation (continued) Demo of prepping, loading, and running the Illumina MiSeq	Carmen Li Dr. Christine Sharp
11:00 – 12:00	Lunch & Illumina presentation: Nextera DNA Flex	Dr. Andrew Warkman
12:00 - 15:45	Troubleshooting metagenome library prep. Presentation on troubleshooting metagenome library prep and sequencing Case studies Q&A session	Carmen Li Dr. Christine Sharp
15:45 – 16:00	Closing Remarks	Dr. Marc Strous
16:00 - 18:00	Wetlab closing and networking reception with EBG