MetaAmp Version 3.0 - OTU based amplicon analysis

MetaAmp is designed to to analyze the amplicon sequening of conserved marker genes (e.g. 16S, 18S rRNA) from complex microbial communities. Its OTU analysis component clusters amplicon sequences into operational taxonomic units (OTUs) using UPARSE. MetaAmp produces alpha and beta diversity based on the inferred OTUs for the the input microbial communities. MetaAmp also assigns taxonomy to each OTU and generates taxonomic profiles. MetaAmp accepts single or paired-end sequencing data in fasta or fastq format from various sequencing platforms (not Nanopore).

To explore the MetaAmp OTU analysis outputs, please check

MetaAmp OTU Analysis Output Demo
Analysis Name:
Email address:
Sequence format:
Fastq   Fasta
Sequencing type:
Paired-end   Single
Upload archived sequence file (demultiplexed):
Upload mapping file (text format):
Forward amplicon primers (5'->3'):
Reverse amplicon primers (5'->3'):
Similarity cutoff:

  

Paired end merging options

Minimum length of overlap:
Maximum number of mismatches in the overlap region:

Quality filtering options

Maximum number of differences to the primer sequence:
Maximum number of expected errors:
Trim amplicon to a fixed length:
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