MetaAmp Version 3.0 - ASV based amplicon analysis

MetaAmp is designed to to analyze the amplicon sequening of conserved marker genes (e.g. 16S, 18S rRNA) from complex microbial communities. Its ASV analysis component infers amplicon sequence variants (ASVs) of the input communities using DADA2. It produces alpha and beta diversity based on the inferred ASVs for the the input microbial communities. MetaAmp also assigns taxonomy to each ASV and generates taxonomic profiles. MeteAmp ASV component accepts single-read or paired-end sequencing in fastq format from various sequencing platforms (Not nanopore).

To explore the MetaAmp ASV analysis outputs, please check

MetaAmp ASV Analysis Output Demo
Analysis Name:
Email address:
Sequencing type:
Paired-end   Single
Upload archived sequence file (demultiplexed):
Upload mapping file (text format):
Forward amplicon primers (5'->3'):
Reverse amplicon primers (5'->3'):
Maximum number of differences to the primer sequence:
MaxN:

  

Single-end reads: quality filtering options

MaxEE:
TruncQ:
TruncLen:

Paired-end reads: quality filtering options

TruncQ:
Read 1: Read 2:
TruncLen:
Read 1: Read 2:
MaxEE:
Read 1: Read 2:

Paired-end reads: denoised pair of forward and reverse reads mergering options

Minimum length of overlap:
Maximum number of mismatches in the overlap region:
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